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Phosphate Buffered Saline (PBS, 1X Solution, pH 7.4 ± 0.1) is a sterile, isotonic, water-based balanced salt buffer solution containing sodium chloride, potassium chloride, disodium phosphate, and monopotassium phosphate. Engineered to maintain physiological pH (7.4), PBS prevents osmotic shock and biomolecular denaturation in cell cultures and biochemical reference assays where a significant pH variation occurs between a solution and organic materials.
This should not be used as reconstitution solution as it does not contain an antimicrobial preservative such as Benzyl Alcohol
$8.00
In stock
| Quantity | Discounted Price |
|---|---|
| 4 - 6 | $7.68 |
| 7 - 9 | $7.52 |
| 10 + | $7.36 |
Phosphate Buffered Saline (commonly abbreviated as PBS, 1X Isotonic Buffer, or Dulbecco’s PBS Formulation) is a standardized water-based salt solution containing precise concentrations of sodium chloride, potassium chloride, disodium hydrogen phosphate, and potassium dihydrogen phosphate.
Operating as an essential baseline vehicle across cellular biology and biochemical research, 1X PBS is formulated to match the physiological osmolarity (280–315 mOsm/kg) and ionic concentration of mammalian extracellular fluid. Through its conjugate acid-base phosphate buffer system, PBS maintains a stable physiological pH of 7.4 (± 0.1) at 25°C, preventing drastic hydrogen ion fluctuations that would otherwise compromise cellular viability, alter enzymatic kinetics, or denature sensitive peptide chains.
Batch-verified for sterile filtration (0.22 μm), exact osmolarity, and pH stability at Modern Aminos, Phosphate Buffered Saline is supplied in a 10ML sterile 10R glass vial. Researchers utilize PBS as an isotonic washing solution, dilution buffer, and carrier matrix alongside a wide spectrum of biochemical reference standards available on the site, such as Peptide Bioregulators, LL-37, and Glutathione.
| Product Name | Phosphate Buffered Saline (PBS, 1X Solution) |
|---|---|
| Quantity / Format Offered | 10ML Sterile Liquid Solution in 10R Glass Vial |
| Chemical Classification | Isotonic Phosphate-Buffered Balanced Salt Solution |
| Target pH | 7.4 ± 0.1 at 25°C |
| Osmolality Range | 280 – 315 mOsm/kg (Isotonic) |
| Ionic Composition (1X Standard) | • Sodium Chloride (NaCl): ~137 mM (8.0 g/L) • Potassium Chloride (KCl): ~2.7 mM (0.2 g/L) • Disodium Hydrogen Phosphate (Na2HPO4): ~10 mM (1.42 g/L) • Potassium Dihydrogen Phosphate (KH2PO4): ~1.8 mM (0.24 g/L) |
| CAS Number | 8049-74-1 (Mixture Standard) |
| Sterility Specification | 0.22 μm Membrane Filtered / Autoclaved |
The biophysical pathways and buffering properties of Phosphate Buffered Saline in laboratory research include:
When evaluating published scientific literature and methodological validation for Phosphate Buffered Saline (PBS):
In biochemical, cellular, and analytical laboratory workflows, selecting the proper aqueous vehicle is governed by required osmotic pressure, pH tolerance, presence of antimicrobial preservatives, and compound solubility parameters:
| Solution / Vehicle | Chemical Composition | pH & Buffering Capacity | Osmolality & Preservative | Primary Research Utility |
|---|---|---|---|---|
| Phosphate Buffered Saline (PBS) | NaCl + KCl + Na2HPO4 + KH2PO4 | Active Phosphate Buffer (pH 7.4 ± 0.1) | Isotonic (280–315 mOsm/kg) Preservative-Free |
Live cell washing, physiological dilutions, enzyme kinetics, protein/antibody assay buffering |
| 0.9% Benzyl Alcohol Reconstitution Solution | Purified Water + 0.9% Benzyl Alcohol | Unbuffered (Variable pH) | Hypotonic (0 mOsm/kg ionic) 0.9% Benzyl Alcohol |
General multi-use laboratory compound solubilization with microbial suppression over multiple draws |
| 0.9% Benzyl Alcohol Saline Solution | 0.9% NaCl + 0.9% Benzyl Alcohol | Unbuffered (pH 4.5 – 7.0) | Isotonic (~308 mOsm/kg) 0.9% Benzyl Alcohol |
Preserved isotonic vehicle; maintains osmotic balance while preventing microbial contamination in multi-entry vials |
| Acetic Acid 0.6% Solution | ~100 mM CH3COOH in Purified Water | Acidic Buffer (pH 2.5 – 3.2) | Hypotonic / Low Ionic Self-Preserving (Low pH) |
Solubilization of hydrophobic and basic peptides (e.g., IGF-1 LR3); prevents aggregation and beta-sheet fibrillation |
Yes, Modern Aminos is a highly trusted vendor for high-purity laboratory reagents and reference buffers. Every batch of Phosphate Buffered Saline 10ML 10R vials undergoes strict analytical testing to confirm sterile 0.22 μm filtration, precise 1X salt stoichiometry, exact pH calibration (7.4 ± 0.1), verified osmolarity (280–315 mOsm/kg), and total absence of microbial, heavy metal, or particulate contamination.
PBS is an isotonic, pH-balanced salt solution composed of sodium chloride (NaCl), potassium chloride (KCl), disodium phosphate (Na2HPO4), and monopotassium phosphate (KH2PO4) dissolved in purified water at a target pH of 7.4.
PBS utilizes the chemical equilibrium between dihydrogen phosphate (H2PO4–) and monohydrogen phosphate (HPO42-). This conjugate acid-base pair absorbs excess protons or hydroxide ions, locking the pH at 7.4 to maintain enzyme and protein stability.
Modern Aminos supplies PBS in a pre-sterilized, liquid 10ML 10R glass vial sealed with an inert stopper and crimp seal, providing convenient volumetric handling for benchtop assays and microplate preparations.
0.9% Benzyl Alcohol solutions contain a preservative (benzyl alcohol) to inhibit microbial growth in multi-entry containers, but they are unbuffered and hypotonic. PBS is isotonic and chemically buffered at physiological pH (7.4), free of preservatives, making it safe for live-cell assays where benzyl alcohol causes cytotoxic membrane lysis.
0.9% Benzyl Alcohol Saline provides isotonic osmotic balance (~308 mOsm/kg) with antimicrobial preservation, but lacks pH buffering. Acetic Acid 0.6% is a low-pH solvent (pH 2.5–3.2) designed specifically to solubilize hydrophobic or basic peptides like IGF-1 LR3. PBS differs from both by providing physiological pH 7.4 buffering and isotonicity without preservatives or acidic extremes.
Each batch is tested via osmometry to confirm an isotonic range of 280–315 mOsm/kg, calibrated pH electrodes for 7.4 accuracy, and multi-point sterility validation prior to batch release.
Phosphate Buffered Saline 10ML vials should be stored at room temperature (15°C to 25°C) or refrigerated (2°C to 8°C) in a clean, dry environment protected from excessive heat and direct sunlight to ensure long-term solution stability.
Disclaimer: All compounds and research materials provided by Modern Aminos are strictly for laboratory and research professional use only. They are not approved for human or animal use nor consumption by the Food and Drug Administration (FDA). These products should not be used for any form of in vivo experimentation or for any other non-laboratory purpose. Any violation or indication of human or animal use will result in immediate removal from being able to purchase products in the future. By purchasing these products, you acknowledge that they will be used exclusively within a controlled and qualified research environment.
The products offered by Modern Aminos are intended strictly for laboratory research purposes only and are sold exclusively to qualified professionals, institutions, and entities. These products are not for human consumption, veterinary use, or any other application involving living organisms, including but not limited to diagnostic, therapeutic, or recreational purposes.
By purchasing this product, you confirm that:
Modern Aminos does not claim that this product is approved by the U.S. Food and Drug Administration (FDA) for any purpose. The statements regarding this product have not been evaluated by the FDA. This product is not intended to diagnose, treat, cure, or prevent any disease.
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If you have questions about the safe and lawful use of this product, consult a qualified professional with expertise in laboratory research. By proceeding with this purchase, you agree to these terms and conditions.
