BAM15 is a mitochondrial-selective protonophore uncoupler engineered to discharge the inner mitochondrial membrane proton gradient without depolarizing the cell plasma membrane. By uncoupling oxidative phosphorylation from ATP synthase, BAM15 elevates mitochondrial oxygen consumption rate (OCR), accelerates fatty acid oxidation, and suppresses mitochondrial reactive oxygen species (ROS) generation across broad concentration windows.
SOLD FOR RESEARCH PURPOSES ONLY
$159.00
In stock
| Quantity | Discounted Price |
|---|---|
| 3 - 5 | $151.05 |
| 6 - 8 | $147.87 |
| 9 + | $143.10 |
BAM15 (known chemically as N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine) is a pioneer selective mitochondrial protonophore uncoupler. Developed to overcome the severe plasma membrane depolarization and narrow therapeutic windows of legacy uncouplers (such as 2,4-dinitrophenol / DNP and FCCP), BAM15 selectively translocates protons across the inner mitochondrial membrane while remaining completely inert at the plasma membrane barrier.
By dissipating the proton motive force without arresting cellular ATP production, BAM15 forces cells to increase nutrient oxidation rates to maintain basal energy demands. This stimulates robust oxygen consumption rates (OCR), drives mitochondrial lipid oxidation, and reduces oxidative stress by suppressing mitochondrial superoxide generation.
Batch-verified for high analytical purity at Modern Aminos, BAM15 is offered as a standardized reference material for cell culture and biochemical bioenergetics research. Researchers evaluating uncoupled cellular respiration, fatty acid oxidation flux, and anti-obesity metabolic mechanisms evaluate BAM15 alongside complementary metabolic reference standards available on the site, such as SLU-PP-332, DADA (Diisopropylamine Dichloroacetate), AICAR, and MOTS-C.
| Compound Name | BAM15 (Mitochondrial Protonophore Uncoupler) |
|---|---|
| Quantity / Formats Offered | High-Purity Dry Reference Powder / Solid Unit Material |
| Chemical Structure | Fluorinated Oxadiazolopyrazinediamine Derivative |
| Synonyms / Alt Names | BAM15, BAM-15, N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine |
| CAS Number | 210344-95-9 |
| Chemical Formula | C16H10F2N6O |
| Molecular Weight | 340.29 g/mol |
| IUPAC Name | N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine |
| InChIKey | InChIKey=VFUQOQAWLKIXCA-UHFFFAOYSA-N |
| SMILES Code | C1=CC=C(C(=C1)NC2=NC3=NON=C3N=C2NC4=CC=CC=C4F)F |
The biochemical pathways and cellular targets include:
Evaluating research efficacy and published scientific literature:
To evaluate uncoupling mechanisms, plasma membrane safety, and bioenergetic efficiency, researchers compare BAM15 against alternative reference standards available at Modern Aminos:
In cellular respiration and mitochondrial bioenergetics research, protonophore selectivity dictates toxicity profiles:
| Compound / Reference | Chemical Structure & Class | Core Mechanism of Action | Research Focus & Profile |
|---|---|---|---|
| BAM15 | Fluorinated Oxadiazolopyrazinediamine Uncoupler | Discharges mitochondrial inner membrane proton gradient to uncouple OXPHOS from ATP synthesis without plasma membrane effect | Focuses on plasma membrane-sparing uncoupled respiration, oxygen consumption rate (OCR) elevation, and ROS suppression |
| SLU-PP-332 | Pan-ERR Nuclear Receptor Agonist | Binds ERRα/β/γ receptors to recruit PGC-1α and upregulate mitochondrial OXPHOS gene transcription | Focuses on nuclear receptor exercise mimetics, oxidative muscle fiber switching, and de novo mitochondrial biogenesis |
| DADA (Diisopropylamine Dichloroacetate) | PDK Kinase Inhibitor / Vasodilator Salt | Inhibits Pyruvate Dehydrogenase Kinase to maintain active PDH, driving pyruvate into mitochondrial oxidative phosphorylation | Focuses on glucose oxidation, extracellular lactate suppression, and microvascular oxygen delivery assays |
| AICAR | Nucleoside Analog Master AMPK Agonist | Mimics AMP to directly phosphorylate and activate AMP-activated protein kinase (AMPK) independent of cellular ATP levels | Focuses on master metabolic switch activation, glucose uptake, GLUT4 translocation, and metabolic signaling pathways |
Yes, Modern Aminos is a highly trusted vendor for high-purity research chemicals and reference compounds. Every batch undergoes strict third-party analytical testing (including HPLC and Mass Spectrometry) to verify minimum 98%+ chemical purity, correct molecular weight (340.29 g/mol), exact protonophore activity, and complete absence of heavy metals or synthesis impurities.
BAM15 (N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine) is a synthetic fluorinated oxadiazolopyrazinediamine derivative. It is classified chemically as a selective mitochondrial protonophore uncoupler.
BAM15 selectively translocates protons across the inner mitochondrial membrane, dissipating the proton motive force used by ATP synthase. Unlike legacy uncouplers, it lacks affinity for plasma membrane lipids, preserving cell membrane potential and preventing plasma membrane depolarization.
Traditional uncouplers like DNP and FCCP depolarize both mitochondrial and plasma membranes. Plasma membrane depolarization disrupts ion homeostasis, induces calcium influx, and causes cell necrosis at narrow therapeutic ranges. BAM15 avoids this off-target effect.
By uncoupling respiration, this compound forces cells to consume oxygen at maximal rates to maintain energy equilibrium. Concurrently, mild dissipation of inner membrane potential (ΔΨm) reduces electron back-pressure, suppressing mitochondrial reactive oxygen species (ROS) formation.
BAM15 is a chemical protonophore that directly dissipates mitochondrial proton gradients to increase oxygen consumption. SLU-PP-332 is a nuclear receptor agonist that binds ERRs to upregulate transcriptional expression of mitochondrial gene networks over hours and days.
Modern Aminos utilizes High-Performance Liquid Chromatography (HPLC) to confirm active chemical purity exceeding 98% and Mass Spectrometry (MS) to verify exact molecular weight (340.29 g/mol) and structural identity prior to batch release.
Dry-fill reference materials should be stored tightly sealed in a cool, dry environment (15°C to 25°C) or refrigerated for long-term preservation. Protect from direct heat, light exposure, and atmospheric moisture to maintain solid-state chemical stability.
Disclaimer: All compounds and research materials provided by Modern Aminos are strictly for laboratory and research professional use only. They are not approved for human or animal use nor consumption by the Food and Drug Administration (FDA). These products should not be used for any form of in vivo experimentation or for any other non-laboratory purpose. Any violation or indication of human or animal use will result in immediate removal from being able to purchase products in the future. By purchasing these products, you acknowledge that they will be used exclusively within a controlled and qualified research environment.
The products offered by Modern Aminos are intended strictly for laboratory research purposes only and are sold exclusively to qualified professionals, institutions, and entities. These products are not for human consumption, veterinary use, or any other application involving living organisms, including but not limited to diagnostic, therapeutic, or recreational purposes.
By purchasing this product, you confirm that:
Modern Aminos does not claim that this product is approved by the U.S. Food and Drug Administration (FDA) for any purpose. The statements regarding this product have not been evaluated by the FDA. This product is not intended to diagnose, treat, cure, or prevent any disease.
The buyer assumes full responsibility for ensuring the safe handling, storage, and use of this product. Modern Aminos is not liable for any damages, direct or indirect, resulting from improper handling, storage, or unauthorized use of this product. Furthermore, Modern Aminos reserves the right to refuse sales to any individual or entity suspected of misusing its products.
If you have questions about the safe and lawful use of this product, consult a qualified professional with expertise in laboratory research. By proceeding with this purchase, you agree to these terms and conditions.
