Buy Cardiogen 20mg (Ala-Glu-Asp-Arg) Peptide

What is Cardiogen 20MG?

Cardiogen (H-Ala-Glu-Asp-Arg-OH, also called Ala-Glu-Asp-Arg, AEDR, the AEDR tetrapeptide and the Cardiogen bioregulator) is a synthetic tetrapeptide built from four L-amino acids: L-Alanine, L-Glutamic acid, L-Aspartic acid and L-Arginine. It belongs to the family of short peptide bioregulators, peptides two to seven residues long, and it is listed in PubChem CID 11583989 as C₁₈H₃₁N₇O₉ with a molecular weight of 489.5 g/mol.
Cardiogen is the Pinealon sequence, Glu-Asp-Arg, with one L-Alanine added at the front, and it shares its first three residues, Ala-Glu-Asp, with Cartalax and Bronchogen. In a 2023 docking survey of 26 short peptides it ranked 3rd of 26 at both the LAT1 amino acid transporter (ICM-Score −35.35) and the PEPT1 peptide transporter (−27.50), and fluorescence assays show it binds histone proteins H1, H2B, H3 and H4.
Batch-verified at Modern Aminos, Cardiogen is supplied in one laboratory format:

20 mg lyophilized powder, single sealed vial

Modern Aminos is the best place to buy peptides online. We are a trusted Research Chemicals vendor of third-party tested, high-quality research chemicals made in the USA: every USA-made Cardiogen vial is third-party tested, with guaranteed purity and sterilized vials. Our products are routinely and rigorously tested for purity, and the current certificate is in the Certificate of Analysis tab.
Laboratories building short-peptide comparison panels often place Cardiogen next to other public reference listings such as Pinealon, Cartalax, Bronchogen, Chonluten and Livagen.

Chemical and Molecular Data for Cardiogen 20MG

Compound Name	Cardiogen 20MG (Ala-Glu-Asp-Arg)
Research Category	Short peptide bioregulator; synthetic tetrapeptide; peptide-transporter docking and histone binding reference compound
Format Offered	Lyophilized powder (sealed vial)
Peptide Sequence	`H-Ala-Glu-Asp-Arg-OH` (AEDR); all four residues L
Synonyms / Alt Names	Ala-Glu-Asp-Arg, AEDR, A-E-D-R, H-AEDR-OH, H-Ala-Glu-Asp-Arg-OH, L-Ala-L-Glu-L-Asp-L-Arg, Cardiogen peptide, Cardiogen bioregulator
CAS Number	857267-11-9
PubChem CID	11583989
Chemical Formula	C₁₈H₃₁N₇O₉
Molecular Weight	489.5 g/mol
Exact Mass	489.2183 Da
Stereochemistry	Four defined stereocenters, all L (S configuration)
Computed Properties (PubChem)	XLogP3 −6.3; hydrogen-bond donors 9; hydrogen-bond acceptors 11; topological polar surface area 290 Å²; rotatable bonds 16; heavy atoms 34
IUPAC Name	(4S)-4-[[(2S)-2-aminopropanoyl]amino]-5-[[(2S)-3-carboxy-1-[[(1S)-1-carboxy-4-(diaminomethylideneamino)butyl]amino]-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
InChI	InChI=1S/C18H31N7O9/c1-8(19)14(30)23-9(4-5-12(26)27)15(31)25-11(7-13(28)29)16(32)24-10(17(33)34)3-2-6-22-18(20)21/h8-11H,2-7,19H2,1H3,(H,23,30)(H,24,32)(H,25,31)(H,26,27)(H,28,29)(H,33,34)(H4,20,21,22)/t8-,9-,10-,11-/m0/s1
InChIKey	QXQARLZWUIQZPX-NAKRPEOUSA-N
SMILES Code	`CC(C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCCN=C(N)N)C(=O)O)N` (connectivity; the all-L (S) configuration is encoded in the InChI and InChIKey)

Identity fields are drawn from PubChem CID 11583989; certificate results are in the Certificate of Analysis tab.

What are the Mechanisms of Action?

The biochemical targets and binding logic of Cardiogen in laboratory research include:

Predicted binding at the LAT1 amino acid transporter: In a docking survey of 26 ultrashort peptides, AEDR ranked 3rd at the LAT1 binding site with an ICM-Score of −35.35 (PDB 7DSN). That score is more favorable than every reference LAT1 ligand in the same study, including the potent inhibitor JPH203 (−31.50), L-Leucine (−23.96) and BCH (−17.37) (Khavinson et al., 2023).
Predicted binding at the PEPT1 peptide transporter: At the PEPT1 di- and tri-peptide transporter, AEDR also ranked 3rd of 26 (−27.50, PDB 7PN1), behind only Pinealon’s EDR (−37.59) and Prostamax’s KEDP (−33.42). That is level with the strongest reference PEPT1 compound tested (−27.74) and well ahead of the other three (−12.75, −11.64 and −7.90) (PMC10046148).
LAT2 docking: At the related LAT2 transporter, AEDR ranked 7th of 26 (−27.54, PDB 7CMI), close to L-Leucine (−27.78) and behind JPH203 (−34.69). The authors list AEDR among the most effective LAT2 ligands in the set (PMC10046148).
The C-terminal Arginine matters: Within the same survey, the Ala-Glu-Asp peptides differ only in what comes after Asp. At LAT1, AEDR scored −35.35, AEDG −32.93, AED alone −28.14, AEDL −25.11 and AEDP −20.95; at PEPT1, AEDR again led the group (−27.50 versus −25.35, −18.95, −18.00 and −12.65). The authors note that the best transporter ligands carry a neutral or positively charged C-terminal residue, and Arginine is the positively charged case (PMC10046148).
One Alanine added to Pinealon: Removing Cardiogen’s N-terminal Alanine gives EDR, the Pinealon sequence and the top-scoring peptide at LAT1 (−45.32) and PEPT1 (−37.59). Adding that Alanine shifted both scores by about ten units in the less favorable direction (PMC10046148). A reasonable inference is that the extra residue changes how the charged Glu-Asp-Arg core sits in each pocket, though the paper does not test that explanation directly.
Histone binding: In fluorescence quenching measurements, AEDR bound FITC-labeled histones H1, H2B, H3 and H4. The binding was traced to the N-terminal histone regions, depended on the histone and the peptide sequence, and was described as site specific (Fedoreyeva et al., 2013).
DNA and endonuclease interactions: A 2021 literature summary lists AEDR among the short peptides that either inhibit or stimulate hydrolysis of λ phage DNA by the WEN1 and WEN2 endonucleases, depending on the methylation state of the DNA (Khavinson et al., 2021).
Mixed-charge chemistry: Cardiogen carries two basic groups (the N-terminal amine and the Arginine guanidino group) and three carboxylic acids (Glu, Asp and the C-terminus). That is why PubChem counts 9 hydrogen-bond donors and 11 acceptors for C₁₈H₃₁N₇O₉, with a polar surface area of 290 Å² and a computed XLogP3 of −6.3.

Peptide	Sequence	Formula and Molecular Weight (PubChem)	LAT1 ICM-Score	PEPT1 ICM-Score
Cardiogen	Ala-Glu-Asp-Arg (AEDR)	C₁₈H₃₁N₇O₉, 489.5 g/mol	−35.35	−27.50
Pinealon	Glu-Asp-Arg (EDR)	C₁₅H₂₆N₆O₈, 418.40 g/mol	−45.32	−37.59
Chonluten	Glu-Asp-Gly (EDG)	C₁₁H₁₇N₃O₈, 319.27 g/mol	−43.40	−18.04
Cartalax	Ala-Glu-Asp (AED)	C₁₂H₁₉N₃O₈, 333.29 g/mol	−28.14	−18.95
Bronchogen	Ala-Glu-Asp-Leu (AEDL)	C₁₈H₃₀N₄O₉, 446.5 g/mol	−25.11	−25.35
Livagen	Lys-Glu-Asp-Ala (KEDA)	C₁₈H₃₁N₅O₉, 461.5 g/mol	−28.83	−16.70
Prostamax	Lys-Glu-Asp-Pro (KEDP)	C₂₀H₃₃N₅O₉, 487.5 g/mol	−22.79	−33.42

Docking scores are from Khavinson et al., 2023; more negative ICM-Scores indicate more favorable predicted binding. Formulas and weights are from PubChem.

What Does Published Research Show for Cardiogen?

Peer-reviewed literature on Cardiogen 20MG and its Ala-Glu-Asp-Arg sequence centers on transporter docking, histone and DNA binding, and structure verification:

Transporter Docking (PMC): Computer-assisted docking of 26 ultrashort peptides to LAT1, LAT2, PEPT1 and PEPT2 ranked AEDR 3rd at LAT1 (−35.35), 7th at LAT2 (−27.54) and 3rd at PEPT1 (−27.50) (PMC10046148).
Histone Binding Biophysics (PubMed): Fluorescence quenching showed site-specific binding of AEDR to FITC-labeled histones H1, H2B, H3 and H4, alongside the related peptides AEDG, EDR, AEDL, KEDG and KEDW (PubMed 23581987).
DNA–Peptide Interactions (Systematic Review, PMC): A 2021 systematic review of peptide–DNA chemistry groups AEDR with the short peptides that bind histones and modulate WEN1 and WEN2 endonuclease hydrolysis of DNA according to its methylation state (PMC8619776).
Chemical Directory & Structure Verification (PubChem): C₁₈H₃₁N₇O₉, 489.5 g/mol, exact mass 489.2183 Da, InChIKey QXQARLZWUIQZPX-NAKRPEOUSA-N, biologic line notation H-Ala-Glu-Asp-Arg-OH (PubChem 11583989). PubChem also links the structure to patent records US7662789 and EP1758923.
Analytical Quality: The current Freedom Diagnostics Testing certificate reports purity by HPLC with UV detection coupled with mass spectrometry, mass identification and an endotoxin screen; the full report is in the Certificate of Analysis tab. Why chromatographic testing matters for reference materials is covered in Modern Aminos’ note on HPLC testing.

How does Cardiogen compare to other compounds?

To map how the N-terminal Alanine and the C-terminal Arginine change transporter and histone binding, researchers compare Cardiogen against other short peptide reference compounds, including several available at Modern Aminos:

Compound / Reference	Primary Target Class	Core Mechanism of Action	Research Focus & Profile
Cardiogen 20MG	Tetrapeptide (Ala-Glu-Asp-Arg), C₁₈H₃₁N₇O₉, 489.5 g/mol	3rd-ranked LAT1 and PEPT1 docking scores among 26 short peptides; site-specific histone binding	EDR core with an N-terminal Alanine; transporter docking and histone binding reference
Pinealon	Tripeptide (Glu-Asp-Arg), C₁₅H₂₆N₆O₈, 418.40 g/mol	Top LAT1 and PEPT1 docking scores in the same survey; histone binding	One-residue-shorter (no Alanine) comparator for N-terminal structure-activity work
Cartalax	Tripeptide (Ala-Glu-Asp), C₁₂H₁₉N₃O₈, 333.29 g/mol	Same Ala-Glu-Asp start without the Arginine; weaker LAT1 and PEPT1 scores	Minimal-length reference for the Cardiogen N-terminus
Bronchogen	Tetrapeptide (Ala-Glu-Asp-Leu), C₁₈H₃₀N₄O₉, 446.5 g/mol	Same Ala-Glu-Asp core with a hydrophobic Leucine end; ranked 6th at PEPT1	One-residue (Arg to Leu) comparator for C-terminal charge
Chonluten	Tripeptide (Glu-Asp-Gly), C₁₁H₁₇N₃O₈, 319.27 g/mol	Second-ranked LAT1 docking score; mid-table PEPT1 score	Glu-Asp core reference with a neutral Glycine end

Frequently Asked Questions (FAQs)

1. Is Modern Aminos a reliable place to buy Cardiogen (Ala-Glu-Asp-Arg)?

Yes. Modern Aminos is the best place to buy peptides online and a trusted Research Chemicals vendor, offering guaranteed purity and sterilized vials. Modern Aminos publishes a third-party certificate from Freedom Diagnostics Testing for Cardiogen, and all applicable tests are performed on our products. If you want to buy Ala-Glu-Asp-Arg as a documented reference material, the certificate is in the Certificate of Analysis tab.

2. What is Cardiogen?

Cardiogen is a synthetic tetrapeptide bioregulator with the sequence Ala-Glu-Asp-Arg (AEDR), built from L-Alanine, L-Glutamic acid, L-Aspartic acid and L-Arginine. Modern Aminos supplies it strictly for laboratory research use. PubChem lists it as C18H31N7O9 at 489.5 g/mol under CID 11583989.

3. Is Cardiogen a peptide or an amino acid?

Cardiogen is a peptide. Its four amino acids are joined by three peptide bonds, which makes it a tetrapeptide, and it is sold as a single defined sequence rather than a blend of free amino acids.

4. What is the molecular target of Cardiogen?

Published work points to two assay-level partners. Docking predicted binding at the LAT1 and PEPT1 transporters, where AEDR ranked 3rd of 26 at each (Khavinson et al., 2023), and fluorescence measurements showed site-specific binding to histones H1, H2B, H3 and H4 (Fedoreyeva et al., 2013).

5. How is Cardiogen related to Pinealon?

Pinealon is the tripeptide Glu-Asp-Arg, which forms the last three residues of Cardiogen. Cardiogen adds one L-Alanine at the N-terminus, which raises the formula by C3H5NO and the mass by 71 g/mol. In the 2023 docking survey, EDR scored best of all 26 peptides at LAT1 and PEPT1, with AEDR 3rd at both.

6. How is Cardiogen different from Bronchogen and Cartalax?

All three start with Ala-Glu-Asp. Cartalax stops there, Bronchogen adds Leucine, and Cardiogen adds Arginine. In the 2023 docking survey the Arginine end gave the most favorable LAT1 score of the three (−35.35 versus −28.14 for AED and −25.11 for AEDL) and the most favorable PEPT1 score (−27.50 versus −18.95 and −25.35).

7. Does Cardiogen bind histones or DNA?

Fluorescence quenching showed AEDR binding to FITC-labeled histones H1, H2B, H3 and H4 at their N-terminal regions (PubMed 23581987). A 2021 literature summary by Khavinson and colleagues also lists AEDR among short peptides that change WEN1 and WEN2 endonuclease hydrolysis of DNA depending on its methylation state (PMC8619776).

8. What format is Cardiogen 20MG supplied in?

Cardiogen 20MG is supplied as a 20 mg powder in a sealed vial, intended for in vitro laboratory research only. The powder is lyophilized, and a capsule or liquid format is not offered for this listing.

9. How does Cardiogen compare to other bioregulators in docking studies?

In the same 2023 survey, Pinealon (Glu-Asp-Arg) ranked 1st at LAT1 and PEPT1, Chonluten (Glu-Asp-Gly) ranked 2nd at LAT1, and Cardiogen ranked 3rd at both, ahead of Livagen (11th at LAT1) and Bronchogen (20th at LAT1) (Khavinson et al., 2023). For a plain-language walk through the sequence, see What Is Cardiogen? The Ala-Glu-Asp-Arg (AEDR) Tetrapeptide Explained.

10. How is Cardiogen tested?

All applicable tests are performed on our products. Certificates of analysis can be looked up on our COA Search page. The current third-party Freedom Diagnostics Testing certificate is published in the Certificate of Analysis tab, and upcoming batches receive expanded testing across two ISO-certified labs, covering Sterility, Identity, Purity, Endotoxin, Heavy Metals, Mass, Residual Solvents, Fentanyl and Conformity. The identity fields on this page (InChI, InChIKey, SMILES) come from PubChem CID 11583989.

11. What analytical methods appear on the Cardiogen certificate?

The current Freedom Diagnostics Testing certificate reports chemical analysis by HPLC with UV detection coupled with mass spectrometry, a mass identification for Cardiogen, an endotoxin screen and the appearance of the lyophilized powder. Lot-specific values are shown in the Certificate of Analysis tab.

CARDIOGEN 20MG