BRONCHOGEN 20MG - Modern Aminos - 3rd-Party Tested | 99% Purity

Q: How does Bronchogen compare to Chonluten?

Chonluten is the tripeptide Glu-Asp-Gly (C11H17N3O8, 319.27 g/mol), while Bronchogen is the tetrapeptide Ala-Glu-Asp-Leu (446.5 g/mol). Both carry the Glu-Asp core. In the same docking survey, Glu-Asp-Gly scored −43.40 at LAT1 against −25.11 for Bronchogen, but Bronchogen scored better at PEPT1 (−25.35 against −18.04).

Chemical and Molecular Data

What is Bronchogen 20MG?

Bronchogen is a synthetic short peptide bioregulator with the sequence Ala-Glu-Asp-Leu (AEDL), a tetrapeptide from the Khavinson family of short peptides. Modern Aminos offers it as 20 mg of white lyophilized powder for DNA binding, histone binding and transporter docking research.

Bronchogen shares its first three residues, Ala-Glu-Asp, with Cartalax and Cardiogen. Its fourth residue, L-Leucine, gives it a neutral, hydrophobic C-terminal side chain. That small difference makes it a useful comparison point for studying how one residue changes DNA, histone and transporter binding.

Modern Aminos is the best place to buy peptides online. We are a trusted Research Chemicals vendor of third-party tested, high-quality research chemicals made in the USA. Each Bronchogen lot is batch-verified for high analytical purity, with guaranteed purity and sterilized vials. Our products are routinely and rigorously tested for purity, and the current certificate is in the Certificate of Analysis tab.

Chemical and Molecular Data

Compound Name	Bronchogen (Ala-Glu-Asp-Leu)
Research Category	Synthetic tetrapeptide; Khavinson short peptide bioregulator
Format Offered	20 mg lyophilized powder, sealed 2R glass vial
Peptide Sequence	H-Ala-Glu-Asp-Leu-OH (AEDL)
Synonyms	Bronchogen; AEDL; Ala-Glu-Asp-Leu; L-Alanyl-L-glutamyl-L-aspartyl-L-leucine
CAS Number	857267-12-0
PubChem CID	11690869
Chemical Formula	C₁₈H₃₀N₄O₉
Molecular Weight	446.5 g/mol
Exact Mass	446.2013 Da
Stereochemistry	4 defined stereocenters, all L (S)
Computed Properties (PubChem)	XLogP3 −3.8; TPSA 225 Å²; 7 H-bond donors; 10 H-bond acceptors; 14 rotatable bonds
IUPAC Name	(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-aminopropanoyl]amino]-4-carboxybutanoyl]amino]-3-carboxypropanoyl]amino]-4-methylpentanoic acid
InChI	InChI=1S/C18H30N4O9/c1-8(2)6-12(18(30)31)22-17(29)11(7-14(25)26)21-16(28)10(4-5-13(23)24)20-15(27)9(3)19/h8-12H,4-7,19H2,1-3H3,(H,20,27)(H,21,28)(H,22,29)(H,23,24)(H,25,26)(H,30,31)/t9-,10-,11-,12-/m0/s1
InChIKey	OFZRHTIWKZWPQF-BJDJZHNGSA-N
SMILES (connectivity)	CC(C)CC(C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CCC(=O)O)NC(=O)C(C)N

What are the Mechanisms of Action?

Published Bronchogen research sits at the molecular level: how the peptide interacts with DNA, with histone proteins and with transporter binding sites in computer models. Each point below is tied to a citation in the next section.

DNA duplex thermostability: In differential scanning microcalorimetry, Bronchogen raised the DNA melting temperature by 3.1 °C at peptide-to-base-pair ratios of 0.01 to 0.055, with no further increase at higher ratios. Melting enthalpy was unchanged from 0.01 to 1.0, and the effect was not AT- or GC-specific. The authors described strong binding with both strands, mainly through the nitrogen bases.
Predicted DNA binding site: Molecular docking of DNA-peptide complexes placed Ala-Glu-Asp-Leu, and its C-terminal fragment Glu-Asp-Leu, at the ctcc sequence. Reasonable inference: because both peptides share the Glu-Asp-Leu end, that end appears to carry the predicted contact.
Histone binding: Fluorescence quenching assays found Ala-Glu-Asp-Leu among six short peptides that bound FITC-labeled histones H1, H2B, H3 and H4, mainly at their N-terminal regions.
Transporter docking: In a docking survey of 26 short peptides, Bronchogen ranked 6th of 26 at PEPT1 (ICM-Score −25.35), inside the −7.90 to −27.74 range the authors report for known PEPT1 inhibitors. It ranked 20th at LAT1 (−25.11), slightly stronger than L-Leucine (−23.96), and 22nd at LAT2 (−18.88).
Charge and polarity: Bronchogen carries three carboxylic acid groups (Glu, Asp and the C-terminus) and one free amine, which makes it net acidic near neutral pH. PubChem computes an XLogP3 of −3.8 and a TPSA of 225 Å². Reasonable inference: the Leucine isobutyl side chain makes it less polar than Cardiogen, whose C-terminal Arginine adds a positive charge.

What do Preclinical & Academic Studies Show for Bronchogen?

Bronchogen has published DNA calorimetry, DNA docking, histone fluorescence and transporter docking data. The findings below are limited to chemistry, binding and assay results.

DNA thermostability: Monaselidze and colleagues used differential scanning microcalorimetry to measure a 3.1 °C rise in DNA melting temperature with Bronchogen, with no change in melting enthalpy (PubMed 21240358).
DNA sequence docking: Khavinson and colleagues modeled DNA-peptide complexes for 19 short peptides and placed Ala-Glu-Asp-Leu and Glu-Asp-Leu at the ctcc sequence (PubMed 27909961).
Histone binding: Fedoreyeva and colleagues reported site-specific binding of Ala-Glu-Asp-Leu to histones H1, H2B, H3 and H4 by fluorescence quenching (PubMed 23581987).
Transporter docking: Khavinson, Linkova, Rudskoy and Petukhov docked 26 short peptides into LAT1, LAT2, PEPT1 and PEPT2 structures. Bronchogen scored −25.11 at LAT1, −18.88 at LAT2 and −25.35 at PEPT1 (Biomolecules, 2023).
Identity reference: PubChem lists Bronchogen as CID 11690869, C₁₈H₃₀N₄O₉, 446.5 g/mol (PubChem CID 11690869).
Analytical quality: The current Freedom Diagnostics Testing certificate reports purity by HPLC with UV detection coupled with mass spectrometry, mass identification and endotoxin results. Read more about why HPLC testing matters.

How does Bronchogen compare to other short peptide bioregulators?

Bronchogen belongs to a family of Glu-Asp short peptides. The table compares sequence and the docking scores reported in the same 26-peptide survey (Biomolecules, 2023). More negative ICM-Scores mean stronger predicted binding.

Peptide	Sequence	Formula / MW	LAT1	LAT2	PEPT1
Bronchogen	Ala-Glu-Asp-Leu (AEDL)	C₁₈H₃₀N₄O₉ / 446.5	−25.11	−18.88	−25.35
Cardiogen	Ala-Glu-Asp-Arg (AEDR)	C₁₈H₃₁N₇O₉ / 489.5	−35.35	−27.54	−27.50
Cartalax	Ala-Glu-Asp (AED)	C₁₂H₁₉N₃O₈ / 333.3	−28.14	−23.64	−18.95
Chonluten	Glu-Asp-Gly (EDG)	C₁₁H₁₇N₃O₈ / 319.3	−43.40	−26.82	−18.04
Pinealon	Glu-Asp-Arg (EDR)	C₁₅H₂₆N₆O₈ / 418.4	−45.32	−32.04	−37.59
Vesugen	Lys-Glu-Asp (KED)	C₁₅H₂₆N₄O₈ / 390.4	−24.79	−20.09	−16.42

Bronchogen (Ala-Glu-Asp-Leu) vs. Cardiogen, Cartalax, Chonluten and Vesugen

Bronchogen and Cardiogen differ only at the fourth residue. Swapping basic Arginine for neutral Leucine weakens the predicted LAT1 and LAT2 scores but leaves PEPT1 close. The authors list both peptides among the most effective transporter ligands in the set, and both fit their pattern of acidic Glu and Asp residues followed by a neutral or positive C-terminus. Next to Cartalax, the added Leucine improves the predicted PEPT1 score from −18.95 to −25.35. Among the Glu-Asp family in this survey, Bronchogen stands out for a PEPT1 score that is stronger than its LAT1 score.

Frequently Asked Questions (FAQs)

Is Modern Aminos a reliable place to buy Bronchogen Bioregulator?

Yes. Modern Aminos is the best place to buy peptides online and a trusted Research Chemicals vendor, offering guaranteed purity and sterilized vials. Modern Aminos publishes a third-party certificate from Freedom Diagnostics Testing for Bronchogen, and all applicable tests are performed on our products. If you want to buy Ala-Glu-Asp-Leu as a documented reference material, the certificate is in the Certificate of Analysis tab.

What is Bronchogen peptide and what is its sequence?

Bronchogen is a synthetic tetrapeptide with the sequence Ala-Glu-Asp-Leu (one-letter code AEDL; written in full as H-Ala-Glu-Asp-Leu-OH). PubChem lists it as C₁₈H₃₀N₄O₉ with a molecular weight of 446.5 g/mol under CID 11690869. For a plain-language walkthrough, see What Is Bronchogen? The Ala-Glu-Asp-Leu (AEDL) Tetrapeptide Explained.

Is Bronchogen a peptide or an amino acid?

Bronchogen is a peptide. It is four amino acids, L-Alanine, L-Glutamic acid, L-Aspartic acid and L-Leucine, joined by three peptide bonds. All four stereocenters are in the L (S) configuration.

What is the mechanism of action of Bronchogen?

Published work describes Bronchogen at the molecular level. In differential scanning microcalorimetry it raised the melting temperature of DNA by 3.1 °C without changing melting enthalpy, and molecular docking places it at the ctcc sequence of DNA. Fluorescence studies report binding to histones H1, H2B, H3 and H4, and a 26-peptide docking survey scored it at the LAT1, LAT2 and PEPT1 transporters.

Is Bronchogen a DNA stabilizer?

That is the best-supported chemical description. In a differential scanning microcalorimetry study, Bronchogen raised the DNA melting temperature by 3.1 °C at peptide-to-base-pair ratios of 0.01 to 0.055, with no further rise at higher ratios. Melting enthalpy did not change, the effect was not specific to AT- or GC-rich regions, and the authors described binding to both DNA strands, mainly through the nitrogen bases.

Does Bronchogen bind histones?

Yes, in fluorescence assays. A 2013 study measured fluorescence quenching of FITC-labeled histones H1, H2B, H3 and H4 and found that Ala-Glu-Asp-Leu was among six short peptides that bound them, with binding located mainly in the histone N-terminal regions.

How does Bronchogen compare to Chonluten?

Chonluten is the tripeptide Glu-Asp-Gly (C₁₁H₁₇N₃O₈, 319.27 g/mol), while Bronchogen is the tetrapeptide Ala-Glu-Asp-Leu (446.5 g/mol). Both carry the Glu-Asp core. In the same docking survey, Glu-Asp-Gly scored −43.40 at LAT1 against −25.11 for Bronchogen, but Bronchogen scored better at PEPT1 (−25.35 against −18.04).

How is Bronchogen different from Cardiogen and Cartalax?

All three start with Ala-Glu-Asp. Cartalax stops there as a tripeptide, Cardiogen adds L-Arginine to make Ala-Glu-Asp-Arg, and Bronchogen adds L-Leucine to make Ala-Glu-Asp-Leu. That single fourth residue changes the C-terminal charge: Arginine is basic and Leucine is a neutral, hydrophobic side chain. The Cardiogen guide covers its sibling in detail.

What format and packaging does Modern Aminos offer for Bronchogen?

Bronchogen is supplied as 20 mg of white lyophilized powder in a sealed 2R glass vial. Modern Aminos offers guaranteed purity and sterilized vials.

Is Bronchogen studied for cytokine regulation or surfactant protein research?

Those topics appear in the wider Bronchogen literature, but they come from biological work rather than chemistry or assay data, so this page does not summarize them. The chemistry-level record for Bronchogen covers DNA thermostability, histone binding and transporter docking, described above.

How is Bronchogen tested?

All applicable tests are performed on our products. Certificates of analysis can be looked up on our COA Search page. The current third-party Freedom Diagnostics Testing certificate is published in the Certificate of Analysis tab, and upcoming batches receive expanded testing across two ISO-certified labs, covering Sterility, Identity, Purity, Endotoxin, Heavy Metals, Mass, Residual Solvents, Fentanyl and Conformity.

What analytical methods appear on the Bronchogen certificate?

The current certificate reports purity by HPLC with UV detection coupled with mass spectrometry, mass identification of Bronchogen, an endotoxin result and appearance as a white lyophilized powder. Our guide to HPLC testing explains how that purity method works.